解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Drosophila has helped us understand the genetic mechanisms of pattern formation. Particularly useful have been those organs in which different cell identities and polarities are displayed cell by cell in the cuticle and epidermis (Lawrence, 1992; Bejsovec and Wieschaus, 1993; Freeman, 1997). Here we use the pattern of...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01569
更新日期:2014-02-11 00:00:00
abstract::Proteasome inhibition elicits an evolutionarily conserved response wherein proteasome subunit mRNAs are upregulated, resulting in recovery (i.e., 'bounce-back') of proteasome activity. We previously demonstrated that the transcription factor Nrf1/NFE2L1 mediates this homeostatic response in mammalian cells. We show he...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01856
更新日期:2014-01-01 00:00:00
abstract::A fluorescent imaging technique called fastFISH has been used to track the various steps involved in the transcription of a single DNA molecule. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.02061
更新日期:2014-01-01 00:00:00
abstract::Physiological adaptation to proteotoxic stress in the endoplasmic reticulum (ER) requires retrotranslocation of misfolded proteins into the cytoplasm for ubiquitination and elimination by ER-associated degradation (ERAD). A surprising paradox emerging from recent studies is that ubiquitin ligases (E3s) and deubiquitin...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01369
更新日期:2014-01-01 00:00:00
abstract::Why does a first pregnancy after age 35 increase the risk of breast cancer, and what can be done to combat this? ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.01926
更新日期:2013-12-31 00:00:00
abstract::Obesity produces a chronic inflammatory state involving the NFκB pathway, resulting in persistent elevation of the noncanonical IκB kinases IKKε and TBK1. In this study, we report that these kinases attenuate β-adrenergic signaling in white adipose tissue. Treatment of 3T3-L1 adipocytes with specific inhibitors of the...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01119
更新日期:2013-12-24 00:00:00
abstract::The expression of E-Cadherin, a protein best known for its role in cell adhesion, regulates the onset of embryonic differentiation. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.01839
更新日期:2013-12-17 00:00:00
abstract::Voltage-gated potassium (Kv) channels enable potassium efflux and membrane repolarization in excitable tissues. Many Kv channels undergo a progressive loss of ion conductance in the presence of a prolonged voltage stimulus, termed slow inactivation, but the atomic determinants that regulate the kinetics of this proces...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01289
更新日期:2013-12-10 00:00:00
abstract::Skeletal muscle degeneration is a complication arising from a variety of chronic diseases including advanced cancer. Pro-inflammatory cytokine TNF-α plays a pivotal role in mediating cancer-related skeletal muscle degeneration. Here, we show a novel function for retinoblastoma protein (Rb), where Rb causes sarcomeric ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01228
更新日期:2013-12-03 00:00:00
abstract::As the equipment needed to perform state-of-the-art research in many areas of biology becomes ever more expensive, Eve Marder worries that researchers in less wealthy institutions might be left behind. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01515
更新日期:2013-11-19 00:00:00
abstract::Arm-amputation involves two powerful drivers for brain plasticity-sensory deprivation and altered use. However, research has largely focused on sensory deprivation and maladaptive change. Here we show that adaptive patterns of limb usage after amputation drive cortical plasticity. We report that individuals with conge...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01273
更新日期:2013-11-12 00:00:00
abstract::The gut microbiota of patients recently diagnosed with rheumatoid arthritis is enriched in microbes belonging to the Prevotella genus. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.01608
更新日期:2013-11-05 00:00:00
abstract::Improving the peer review process, overcoming the limitations of print journals and providing open access to the very best work in the life and biomedical sciences are three highlights of our first year. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01516
更新日期:2013-10-15 00:00:00
abstract::Understanding genome to phenotype linkages has been greatly enabled by genomic sequencing. However, most genome analysis is typically confined to the nuclear genome. We conducted a metabolomic QTL analysis on a reciprocal RIL population structured to examine how variation in the organelle genomes affects phenotypic va...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00776
更新日期:2013-10-08 00:00:00
abstract::Cyanobacteria were responsible for the oxygenation of the ancient atmosphere; however, the evolution of this phylum is enigmatic, as relatives have not been characterized. Here we use whole genome reconstruction of human fecal and subsurface aquifer metagenomic samples to obtain complete genomes for members of a new c...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01102
更新日期:2013-10-01 00:00:00
abstract::Cavin-3 regulates metabolism and cell proliferation by coordinating the activities of growth factor signalling cascades. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.01428
更新日期:2013-09-24 00:00:00
abstract::Coat protein complex II (COPII) mediates formation of the membrane vesicles that export newly synthesised proteins from the endoplasmic reticulum. The inner COPII proteins bind to cargo and membrane, linking them to the outer COPII components that form a cage around the vesicle. Regulated flexibility in coat architect...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00951
更新日期:2013-09-17 00:00:00
abstract::Researchers have identified two genes-follistatin and activin-as having an important role in the ability of certain flatworms to identify wounds that require the production of new tissue. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00533
更新日期:2013-09-10 00:00:00
abstract::During cell locomotion and endocytosis, membrane-tethered WASP proteins stimulate actin filament nucleation by the Arp2/3 complex. This process generates highly branched arrays of filaments that grow toward the membrane to which they are tethered, a conflict that seemingly would restrict filament growth. Using three-c...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01008
更新日期:2013-09-03 00:00:00
abstract::Learning more about the cells that enable parasitic worms called schistosomes to reproduce inside snails could lead to new treatments that prevent these parasites from being transmitted to humans. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.01115
更新日期:2013-07-30 00:00:00
abstract::In bacteria, multicellular behaviors are regulated by cell-cell signaling through the exchange of both diffusible and contact-dependent signals. In a multicellular context, Myxococcus cells can share outer membrane (OM) materials by an unknown mechanism involving the traAB genes and gliding motility. Using live imagin...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00868
更新日期:2013-07-23 00:00:00
abstract::Apical secretion from epithelial tubes of the Drosophila embryo is mediated by apical F-actin cables generated by the formin-family protein Diaphanous (Dia). Apical localization and activity of Dia are at the core of restricting F-actin formation to the correct membrane domain. Here we identify the mechanisms that tar...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00666
更新日期:2013-07-09 00:00:00
abstract::While host immune receptors detect pathogen-associated molecular patterns to activate immunity, pathogens attempt to deregulate host immunity through secreted effectors. Fungi employ LysM effectors to prevent recognition of cell wall-derived chitin by host immune receptors, although the mechanism to compete for chitin...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00790
更新日期:2013-07-02 00:00:00
abstract::Photosynthetic starch reserves that accumulate in Arabidopsis leaves during the day decrease approximately linearly with time at night to support metabolism and growth. We find that the rate of decrease is adjusted to accommodate variation in the time of onset of darkness and starch content, such that reserves last al...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00669
更新日期:2013-06-25 00:00:00
abstract::Genetic analyses of 15 species of yeast have shed new light on the divergence of gene regulation during evolution, with significant changes occurring after an event in which a whole genome was duplicated. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.00933
更新日期:2013-06-18 00:00:00
abstract::Understanding how virus capsids assemble around their nucleic acid (NA) genomes could promote efforts to block viral propagation or to reengineer capsids for gene therapy applications. We develop a coarse-grained model of capsid proteins and NAs with which we investigate assembly dynamics and thermodynamics. In contra...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00632
更新日期:2013-06-14 00:00:00
abstract::Termination of messenger RNA translation in Bacteria and Archaea is initiated by release factors (RFs) 1 or 2 recognizing a stop codon in the ribosomal A site and releasing the peptide from the P-site transfer RNA. After release, RF-dissociation is facilitated by the G-protein RF3. Structures of ribosomal complexes wi...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00411
更新日期:2013-06-04 00:00:00
abstract::As the staff of the MRC Laboratory of Molecular Biology settle into their new building in Cambridge, its director Hugh Pelham explains the challenges of living up to its prestigious past. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00856
更新日期:2013-05-28 00:00:00
abstract::Pore-blocking toxins inhibit voltage-dependent K(+) channels (Kv channels) by plugging the ion-conduction pathway. We have solved the crystal structure of paddle chimera, a Kv channel in complex with charybdotoxin (CTX), a pore-blocking toxin. The toxin binds to the extracellular pore entryway without producing discer...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00594
更新日期:2013-05-21 00:00:00
abstract::Dengue Virus (DENV), a flavivirus spread by mosquito vectors, can cause vascular leakage and hemorrhaging. However, the processes that underlie increased vascular permeability and pathological plasma leakage during viral hemorrhagic fevers are largely unknown. Mast cells (MCs) are activated in vivo during DENV infecti...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00481
更新日期:2013-04-30 00:00:00
abstract::Using a clever combination of genetic and biochemical tools researchers have shown that a transcription factor called USF1 has a central role in determining how mutations of the Clock gene manifest themselves in the behaviour of different mouse strains. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.00659
更新日期:2013-04-09 00:00:00
abstract::Brain connectivity maps display a delicate balance between individual variation and stereotypy, suggesting the existence of dedicated mechanisms that simultaneously permit and limit individual variation. We show that during the development of the Drosophila central nervous system, mutual inhibition among groups of nei...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00337
更新日期:2013-03-05 00:00:00
abstract::The clathrin adaptor complex AP2 is thought to be an obligate heterotetramer. We identify null mutations in the α subunit of AP2 in the nematode Caenorhabditis elegans. α-adaptin mutants are viable and the remaining μ2/β hemicomplex retains some function. Conversely, in μ2 mutants, the alpha/sigma2 hemicomplex is loca...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00190
更新日期:2013-03-05 00:00:00
abstract::Sudden changes in the level of a coenzyme called NADPH might be the underlying cause of aging in cells. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00515
更新日期:2013-02-05 00:00:00
abstract::Patterns of spatial positioning of individuals within microbial communities are often critical to community function. However, understanding patterning in natural communities is hampered by the multitude of cell-cell and cell-environment interactions as well as environmental variability. Here, through simulations and ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00230
更新日期:2013-01-22 00:00:00
abstract::Planar cell polarity (PCP) requires the asymmetric sorting of distinct signaling receptors to distal and proximal surfaces of polarized epithelial cells. We have examined the transport of one PCP signaling protein, Vangl2, from the trans Golgi network (TGN) in mammalian cells. Using siRNA knockdown experiments, we fin...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00160
更新日期:2013-01-08 00:00:00
abstract::Single-molecule imaging experiments have shed new light on the methods used by the enzyme RecA to align single- and double-stranded DNA so that double-strand breaks can be repaired. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.00347
更新日期:2012-12-13 00:00:00
abstract::Experiments on Drosophila have shown that the histones that are normally bound to lipid droplets inside cells can be released to provide protection against infection. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.00302
更新日期:2012-11-13 00:00:00
abstract::The new open-access journal eLife has launched, making its first content available in PubMed Central. In addition to publishing science of the highest quality, the journal aims to improve both the peer-review process and the presentation of new research results. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00270
更新日期:2012-10-15 00:00:00